In the present study, an attempt was made to evaluate the impact of different hormones i.e. Indole -3 butyric acid (IBA) and Indole -3 acetic acid (IAA) for the root induction in Gentiana kurroo, a small perennial herb and Cyrtomium caryotideum, a fish-tail holly fern. Both population is fast depleting in their natural habitats due to their high medicinal uses. For the present study growing tip of rhizome was split into 2, 3 and 4 longitudinal parts. Each split contained 1/2, 1/3 or 1/4 of longitudinal section of mother rhizome and above ground part with growing buds. G. kurroo and C. caryotideum two piece rhizome exhibited significantly higher survival percentage than three and four pieces. G. kurroo two and three pieces IBA treated cuttings showed better results than IAA treatments. C. caryotideurris two and three piece cuttings provided best survival under controlled conditions, four piece cuttings thrived better under IAA 300 ppm treatment. Impact of hormones in rhizome-cutting of G. kurroo and C. caryotideum is a new, simple, easy and effective technique for multiplication and conservation of these endangered herb and fern for production of more number of plants for future conservation.