Hill Banana (Cultivar Virupakshi) is one of the highly priced Banana variety of Tamil Nadu grown in the Pulney hills of Dindigul region. Fusarium wilt disease has resulted in reduction in yield and also in the cultivable area. In-vitro propagation of disease free plant is a method to overcome this problem, but interrupted by the excess exudation of phenols.Phenols will darken the tissue culture medium, finally leading to death if not controlled. In the present study, Citric acid and Ascorbic acid at two different concentrations viz., 50 and 100 ppm were used.Explants were either soaked in the antioxidants or they were incorporated into the medium. Soaking of explants for 30 minutes in an anti-oxidant solution prior to their culture caused the maximum reduction in phenol content on 24th DAI from 0.112 per cent and 0.110 to 0.075 per cent and 0.044 per cent at 50 mg and 100 mg concentrations respectively.