Cancer occurs when the cells acquire the property of immortality and keeps on dividing without a halt. The body keeps a check on the normal cell division rate but sometimes this control is lost and it leads to the formation of tumors, which may  be benign or malignant. For cancer studies a part of the tumor is taken from the origin location and cultured till it becomes a stable cell line to be used for in-vitro experimentation. The cytotoxic effect of Doxorubicin and Docetaxel are observed on Human Ovarian and Colon cell lines namely PA1 and SW620. Doxorubucin is classified as an "anthracycline” drugs and is one of the most widely used anticancer drugs. Doxorubicin damages DNA by intercalation of the anthracycline portion, metal ion chelation, or by generation of free radicals.[1] Docetaxel (Taxotere) is of the chemotherapy drug class; taxane, and is a semisynthetic analogue of paclitaxel (Taxol) Docetaxel binds to microtubules reversibly with high affinity. This binding stabilises microtubules and prevents depolymerisation from calcium ions, decreased temperature and dilution.[2] The effectiveness of drugs was observed at different drug concentrations by MTT, (3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay. Transforms, plotting and analysis of the experimental data was done by Graph Pad Prism version 4.03.

KEYWORDS: Doxorubicin, Docetaxel,Anticancer,MTT