Maximal L-glutaminase enzyme production by Brevundimonas diminuta MTCC 8486 (48.4 U/ml) occurred at pH 6.0, 30°C in a sea water based medium supplemented with L-glutamine (1%, w/v), D-glucose (1.5%, w/v), peptone (1%, w/v), and potassium dihydrogen phosphate (1%, w/v) after 28 h of incubation and molecular weight of purified enzyme was 140 kDa.

Keywords : L-Glutaminase, Brevundimonas diminuta, marine bacteria