An attempt was made to study the protease production by immobilized cells of Terediobacter turnirae using different matrices such as calcium alginate, k- carrageenan, polyacrylamide, agar-agar and gelatin gel. Among the different matrices calcium alginate was found to be better than other matrices for higher protease production. Influence of different pH and temperature on production was also studied. The maximum production was found at pH 8 and temperature 60 0C. In the repeated batch fermentation, calcium alginate was more efficient for the production of protease than other matrices.

Keywords: Batch fermentation, Immobilized cells, matrices, calcium alginate, Terediobacter turnirae