Secondary metabolites are particularly produced from plants, fungi and actinomycetes, where as yeasts and animals produce them to a lesser extent. Fungi are well known as prolific sources of distinctive biologically active secondary metabolites. In order to find out the efficacy of secondary metabolites, the crude extract of metabolite is extracted from the fungi. The soil sample is collected and screened for the fungal colonies by using Rose Bengal chloramphenicol media which is specific for the growth of fungi and inhibits the growth of bacteria. Among the colonies obtained, pure culture was made to grow on slants with Potato Dextrose Agar Medium. The structure of fungi was identified by SEM analysis. The fungi was mass cultivated in Potato Dextrose Broth and incubated for 15 days for the release of secondary metabolite. The secondary metabolite was extracted by ethyl acetate and concentrated using Rota vapor. The crude extract obtained was examined for various biological assays. Antimicrobial activity was tested against various bacteria and fungi. Extract was tested for antioxidant scavenging activity. Cytotoxicity was tested upon Brine Shrimps. Larvicidal activity was done to find out the efficacy against mosquito larvae. Then the metabolite was fractionated by using thin layer chromatography.

Key words: Bio-efficacy, Rota vapor, Extraction, Cytotoxicity, Thin Layer Chromatography.