The active compound present in Andrographispaniculata L. - Andrographolide which was isolated and purified using column chromatography, TLC and the characterization of andrographolide was done using FT IR and NMR studies. Furthermore, in vivo and in vitro studies confirm the anti venom property of the plant and the cytotoxic levels determined by the use of cell lines has its major start towards the cellular level study.

Keywords: Andrographispaniculata.L, Andrographolide, medicinal plant