The biodegradation of anthracene, a perilous model Polycyclic aromatic hydrocarbon (PAH) was examined by employing aerobic bacterial strain Alcaligenes faecalis, isolated from contaminated soil. The degradation and growth of the organism on anthracene was experimented at different inoculum concentrations and pH. The optimum conditions were observed to be at 2% (v/v) inoculum concentration and 6.5 pH. The effect on initial substrate concentration proved anthracene as an inhibitory type substrate as the maximum degradation was at 40 mg I"1. Within 40 h and at 30 ° C, the strain metabolized 92.34% of anthracene under optimized conditions. With the simulated and experimental data's, kinetic constants were calculated for Monod, Haldane and competitive inhibition models. Metabolites were identified using High performance liquid chromatography and Gas chromatography mass spectrum for elucidating the biodegradation pathways. Three degradation pathways were proposed, one of which has been demonstrated as a new branch in anthracene degradation pathway because of 9 hydroxy fluorene a novel metabolite. Altogether these results signify the effectual PAH degradation by the promising isolated strain Alcaligenes faecalis MVMB1.