Chlorella pyrenoidosa is a freshwater green alga which is generally utilized as a part of wastewater treatment prepare. The present research expects to investigate the cancer prevention agent movement and anticancer capability of the microalgae Chlorella pyrenoidosa developed from the wastewater created tremendously by the instructive foundation. Regular cancer prevention agents, including shades, phenolics, and tocopherols, were measured in methanolic concentrates of microalgae biomass. The consequences of every test were connected to the substance of regular cancer prevention agents in microalgae biomass. Phenolic mixes were found as real patrons to the cell reinforcement action in all cancer prevention agent tests while carotenoids were found to add to the 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical rummaging movement, ferrous lessening power (FRAP), and ABTS-radical searching limit action. Cytotoxicity of the concentrate was controlled by Methyl thiazolyl tetrazolium (MTT) test. The tumor cells experiencing apoptosis were controlled by Hoechst 3324 fluorescence recoloring. The apoptotic cells were splendid white shaded, which speaks to they have experienced chromatin build-up and atomic fracture.

Key words: Microalgae, wastewater, antioxidant, cytotoxicity, apoptotic