The biosynthesis of Silver nanoparticles (AgNPs) was carried out using the extract of tea fungus (Kombucha consortium). "Kombucha" is commonly known as tea fungus that consists of bacterial and fungal strains. The Kombucha biomass was used for the synthesis of AgNPs. The synthesized AgNPs were characterized by UV-Visible spectroscopy, Dynamic Light Scattering Microscopy (DLSM), FTIR, Atomic Force Microscopy (AFM) and Scanning Electron Microscope (SEM). Biosynthesized AgNPs exhibited antibacterial activity against gram positive and gram negative bacteria (S. aureus and E.coli). The anti-cancer activity was also conducted against human breast cancer cells (MCF-7). The synthesized AgNPs are simple, less expensive and environmental friendly. The AgNPs have wide applications in various fields namely medicine, catalysis and chemistry.

Keywords: Kombucha fungus, Silver nanoparticles, SEM, Antibacterial activity, Anticancer activity.