In recent years, traditional system of medicine has become a topic of global importance wherein the plant metabolites plays an important role as source of pharmaceuticals for various treatments. Herbal medicines are termed as ‘Alternate medicine’ and their of great demand in both developed and developing countries for their wide biological medicinal activity, safety and lesser cost. WHO estimated 80% showed positive correlation between modern therapeutics and traditional herbal medicines. Artemisia nilagirica (clarke) pamp is a sweet-smelling herbaceous perpetual plant has a place with Asteraceae family was usually known as Sage Brush or wormwood and they were developed for its religious significance because of its huge scent. In the present investigation, the leaf extract of Artemisia nilagirica (clarke) pamp was subjected to column chromatographic separation and the fractions were subjected to thin layer chromatography. The Rf value was authenticated to be 0.71 for flavonoids from Artemisia nilagirica. The fractioned compound was subjected to HPLC and the peak was absorbed in the range of 6.623 which represented the compound to be flavonoid.   

Keywords:  Flavonoids, Bioactive compounds, chromatographic techniques, medicinal plant, HPLC