Isozyme can provide effective information on the genetic variations at inter- and intra- populations level. The results reported in this paper concern the genetic variations in Rubia cordifolia L., which is a dye yielding plant. Seeds were collected from five different climatic conditions. Polyacrylamide gel electrophoresis study of eleven enzymes revealed 40 putative alleles.Among the 40 putative alleles, 21 were found in all populations. Allelic frequency and polymorphic loci were determined for each population and the data were further subjected to Cluster analysis. The results revealed greater genetic variations at lower altitude populations than at higher.

Keywords: Alleles, allelic frequency, Isozymes, R. cordifolia, polymorphic loci, dendrogram, genetic variation.