ABSTRACT

Two marine microalgae named nanochloropsis occullata and chaetoceros calcitrans were selected for the study.the crude extract of the antimicrobial compounds were taken by using acetone,butanol,hexane and chloroform: methanol,under percolation method. the crude extracts were tested on aquatic pathogens such as staphylococcus aureus, pseudomonas aeruginosa,vibrio harveyi,vibrio parahaemolyticus and aeromonas hydrophila among the four extract obtained using the said solvents the chlorofoem methanaol resulted maximum inhibitory activity in the extract obtained from both the microalgae. among the four extracts two crude extracts were again purified in column chromatography and got different fraction of compounds. the fractionationed compounds were tested on aquatic pathogen among those some fraction and maximum inhibitory activity the active fractions were analyed for its compound identification through FTIR tecnique. the active compounds were nitrogenous carbonyl and alcohol group.the compounds were exposed to light (1000 lux) to increase the activity.

KEYWORDS: ANTIMICROBIALS,AQUATIC PATHOGENS,ACTIVE COMPOUNDS,FUNCTIONAL GROUP,LIGHT SENSITIVE COMPOUNDS.