The study was conducted to investigate the histo pathological and biochemical studies of the liver of Mystus tengara. The effluents were collected from non point source of River Chambal and the chemical parameters assessed .The COD, BOD, TSS, TS and TDS were much higher than the standard quality. The toxicity of these effluents on Mystus tengara and the LC 50 values were determined. The liver showed vacuolar degeneration, focal areas of necrosis and fibrosis, and aggregations of inflammatory cells. The experimental fishes revealed gradual decrease in albumin and INR. RBC. Plasma level of glucose was also lower in the exposed fish when compared to the control. However high levels of ASP and ALT were observed. In conclusion, the changes observed indicate that hematological and biochemical parameters can be used as an indicator of toxicity related stress in fish exposed to effluents.

Keywords: Effluent, AST, ALT, INR, hematology, Biomarkers