Abstract

In this work the synthesis of stable silver nanoparticles by the bioreduction method from Pseudomonas Aurignosa MTCC2297 is proposed. The culture supernatant of these nanoparticles by reduction of silver ions coming in contact with cell filtrate was fast and formed within minutes. The quantitative formation of nanoparticles is monitored by UV-Vis Spectroscopy and showed a peak of 440-450nm corresponding to the surface plasmon absorbance of nanoparticles. Further characterization was done using FTIR (Fourier Transform Infrared Spectroscopy), XRD (Xray Diffraction Analysis) and AFM (Atomic force Microscopy).FTIR spectroscopy used for quantitative anayses of the reaction products [4]. XRD of silver nanoparticles exhibited 2θ values corresponding to the silver nanocrysta[2]l. AFM shows the morphology and measure size, shape of silver nanoparticles. The average size of the particle diameter was found to be 160-360nm using AFM analysis.

Keywords: UV-Vis Spectroscopy, FTIR (Fourier Transform Infrared Spectroscopy), XRD (Xray Diffraction Analysis) and AFM (Atomic force Microscopy).

Keywords: UV-Vis Spectroscopy, FTIR (Fourier Transform Infrared Spectroscopy), XRD (Xray Diffraction Analysis) and AFM (Atomic force Microscopy).